Marcus Smith will miss England’s second Six Nations game against Wales next Saturday after failing to recover from the calf injury that sidelined him from the narrow win over Italy.
George Ford started at fly-half for England in Rome during a 27-24 victory with debutant Fin Smith coming on for the final 14 minutes.
Marcus Smith will be absent again for the home clash with Wales at Twickenham on February 10.
Ellis Genge has been named in a 36-man squad to assemble ahead of the Wales match having been a late withdrawal from the Italy game with a foot problem.
England have also announced that Leicester lock George Martin will join up with the squad to continue his rehabilitation from injury.